Coding

Part:BBa_K1403005:Design

Designed by: Antonio Villarreal Larrauri   Group: iGEM14_Paris_Bettencourt   (2014-10-06)


JMT CDS with His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 146
    Illegal BamHI site found at 717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silently removed an illegal PstI site from the codon optimised sequence. Added a C-terminus His tag.


Source

CDS of JMT (AY008434.1), codon optimised for E. coli.

References